Technical Data
Fibroblast Growth Factor, Acidic, Recombinant, Human (FGFa, FGF1, aFGF, AFGF, Beta-endothelial cell growth factor, ECGF, ECGFA, ECGFB, ECGF-beta, FGFA, FGF-alpha, GLIO703, HBGF1, HBGF-1, Heparin-binding growth factor 1 precursor)
Molecular Biology Storage: -70CShipping: Dry Ice
FGF acidic, also known as FGF-1 and endothelial cell growth factor, is a member of the FGF family of mitogenic peptides which currently is comprised of at least seven proteins which show 35-55% amino acid sequence conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF acidic has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. As with other FGFs, FGF acidic exhibits considerable species crossreactivity. FGF acidic and FGF basic stimulate the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin.

Biological Activity:
Fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is 10ng/ml (>1x10e5 units/mg).

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O, 0.1% BSA. Aliquot and store at -70C. Reconstituted product is stable for 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Escherichia coli
Purity: ~95% by SDS-PAGE and HPLC analyses. Endotoxin: 1EU/ug (LAL)
Form: Supplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile ddH2O, 0.1% BSA to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.