Technical Data
F4215-05
Fibronectin
Description:
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.

Applications:
Suitable for use as in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunoprecipitation (native and denatured): 2ug/mg protein lysate. Use Protein G.
Optimal dilutions to be determined by the researcher.

Positive Control:
SW156 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.295Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose. Cellular Localization: Connective tissue matrix and basement membrane MW: Non-reduced: 440kD Reduced: 220kD Epitope: Peptide core
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F4215-05X.
Specificity:
Recognizes both the plasma and cellular forms of human fibronectin. Blocks the fibronectin-mediated cell attachment by interfering with the attachment of fibronectin to its receptor on the cell surface: not by disrupting the collagen-fibrinogen interaction.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schoen, R.C., et al., Hybridoma, 1(2): 99-108 (1982). 2. Koch, G.A., et al., Exp. Cell Res., 141: 293-302 (1982).