Technical Data
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix, and blood. It possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization and wound healing.

Suitable for use as in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunoprecipitation (native and denatured): 2ug/mg protein lysate. Use Protein G.
Optimal dilutions to be determined by the researcher.

Positive Control:
SW156 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG10.N.295Affinity Purified
500ul-20CBlue IceHumanMouse
Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose. Cellular Localization: Connective tissue matrix and basement membrane MW: Non-reduced: 440kD Reduced: 220kD Epitope: Peptide core
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See F4215-05X.
Recognizes both the plasma and cellular forms of human fibronectin. Blocks the fibronectin-mediated cell attachment by interfering with the attachment of fibronectin to its receptor on the cell surface: not by disrupting the collagen-fibrinogen interaction.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schoen, R.C., et al., Hybridoma, 1(2): 99-108 (1982). 2. Koch, G.A., et al., Exp. Cell Res., 141: 293-302 (1982).