Technical Data
F4215-46
Fibronectin
Description:
Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane- bound fibronectin receptors on cell surfaces.

Applications:
Suitable for use in Immunohistochemistry, RIA, ELISA, Immunofluorescence and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: 1:80 for immunofluorescent staining of frozen mouse skin and liver tissues.
ELISA: 1:200
Immunofluorescence (IC): Evaluated on NIH/3T3 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Fibronectin extracted and purified from mouse plasma.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added.
Specificity:
Recognizes mouse fibronectin. Less than 0.1% reactivity with mouse Laminin and Collagen Types I, III and IV by radioimmunoassay.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Hanke, M.L. et al., (2012) PLoS ONE 7(8): e42476. doi:10.1371/journal.pone.0042476. 1. Knuesel, I., et al., Neurobiol Aging. 30(5): 697-716 (2009). 2. Murase, S. and Horwitz, A.F., J. Neurosci. 22(9): 3568-3579 (2002).