Technical Data
G1015-02A
GABA (Gamma-aminobutyrIc acid)
Description:
Both neural and neuroendocrine cells are able to synthesize a variety of peptides as well as amino acids that can function either as inhibitory or stimulatory substances in neurotransmission. Thusfar, mostly polyclonal antibodies are available for many of them. In the central nervous system GABA is an early neurotransmitter, which can be detected in the developing nervous system. GABA functions as a neurotransmitter also in the peripheral nervous system. Most studies thusfar published on immunolocalization of GABA containing neurons have been made with rabbit polyclonal antibodies. Clearly, there is an increasing need for monoclonal antibodies against neurotransmitters that would enable double immunolocalization studies.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:500-1: 20,000.
Immunohistochemistry: 1:500-1: 20,000. Works well on frozen sections if perfusion fixed. Paraffin sections require glutaraldehyde in the fixative.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Fusion between myeloma cells and Balb/c spleen cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15G10Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
GABA-coupled to BSA with glutaraldehyde
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity:
Recognizes GABA. Does not react with other amino acid-BSA conjugates with the exception of a weak reaction against b-alanine BSA-conjugate at the highest concentrations used.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jongen-Relo, A.L., et al., J. Comp.Neurol. 408: 237-271 (1999). 2. Miettinen, M., et al., J. Comp. Neurol. 378: 363-378 (1997). 3. Tuunanen, J., et al., Eur. J. Neurosci. 8: 2711-2725 (1996). 4. Pitkänen, A. and Amaral, D., J. Neurosci. 14: 2200 (1994). 5. Erikson, K.S. and Panula, P., J. Comp. Neurol. 345: 526-536 (1994). 6. Szabat, E., et al., Neurosci. 47: 409-420 (1992). 7. Halasy, K., et al., Eur. J. Neurosci. 4: 144 (1992). 8. Airaksinen, M., et al., J. Comp. Neurol. 323: 103 (1992).