Technical Data
G1015-19N3
GABA A receptor delta subunit
Description:
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (4, 5, 8). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (2, 3, 4, 6). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (1) and may also confer differential sensitivity to neurosteroids and to ethanol (7).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 10ul per 50ug lysate
Immunohistochemistry (Frozen): 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Fusion protein from the N-terminus of the rat GABA-A receptor delta subunit. Cellular Localization: muti-pass membrane protein
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Recognizes the 52kD delta subunit of rat brain GABA-A. No known reactivity with other GABA subunits. No homology with other GABA subunits. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Korpi ER, et al. (2002) Neurosci 109:733-743. 2. McKernan RM, et al. (2000) Nature Neurosci 3:587-592. 3. Mehta AK, Ticku MK (1998) Mol Brain Res 67:194-199. 4. Ogris W, et al. (2004) Biochem Pharmacol 68:1621-1629. 5. Olsen RW, Tobin AJ (1990) FASEB 4:1469-1480. 6. Pöltl A, et al. (2003) J Neurochem 87:1444-1455. 7. Wallner M, Hanchar HJ, Olsen RW (2003) Proc Natl Acad Sci (USA) 100:15218-15223. 8. Whiting PJ, et al. (1999) Ann NY Acad Sci 868:645-653.