		Technical Data
G1016	GABA A Receptor beta 2,3

Description:
GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics.
GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml detected the GABA A Receptor beta 2,3 subunits from 20ug of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G1016 (2ug/ml). Proteins were visualized using a Gt xMo IgG (HRP) and ECL.
Immunoprecipitation: 4ug immunoprecipated the GABA A Receptor beta 2,3 subunits from 500ug of rat brain microsomal preparation. Due to interference of the IgG heavy chain, the immunoprecipitated GABA A reseptor complex was confirmed using G1015-06.
Immunohistochemistry: Detects GABA A Receptor beta 2,3 subunits in paraffin embedded and frozen rat brain sections fixed with 4% paraformaldehyde or 1% glutaraldehyde and permeabilized with Triton X-100.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	0.T.52	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~1mg/ml

Immunogen:

Affinity-purified GABAA receptor isolated from bovine brain.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes bovine GABAA Receptor b2 (55kD) and b3 (57kD) subunits. Species Crossreactivity: human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Li, M. & De Blas, A.L., J. Biol. Chem. 272: 16564-16569, 1997. 2. Vitorica, J., et al., J. Neurochem. 54: 187-194, 1990. 3. De Blas, A.L., Mol. Neurobiol. 12: 55-71, 1996. 4. Nusser, Z., et al., J. Neurosci. 18: 1693-1703, 1998. 5. Tretter, V., et al., J. Neurosci. 17: 2728-2737, 1997. 6. McDonald, B.J. and S.J. Moss, Neuropharm. 36: 1377-1385, 1997. 7. Sperk, G., et al., Neuroscience 80: 987-1000, 19.97.