Technical Data
G1040-01
Galactose Oxidase, Liquid (GAO, D-Galactose Oxygen-6-oxidoreductase)
500U
Molecular Biology Storage: -20°CShipping: Blue Ice
Galactose oxidase (GAO) produced by the fungus Dactylium dendroides is used in the quantitative determination of galactose in blood and other biological fluids. Because GAO oxidizes galactose even in polysaccharides it has been used to locate galactose histochemically and to detect and distinguish glycoproteins.

Activity:
30 units per dry weight

% Catalase:
As reported

mg/ml (A280):
As reported

Unit Definition:
1 unit is a change in absorbance at 425 nm of 1.0 per minute at 25ºC, pH 6.0, in a coupled peroxidase/o-tolidine system, using galactose as a substrate.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
9028-79-9

Molecular Weight:
~68kD
Source: Dactylium dendroides
Form: Supplied as a liquid in sodium phosphate and sucose.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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Amaral, D., Kelly-Falcoz, F., and Horecker, B.: Galactose Oxidase of Polyporus circinatus ,Methods in Enzymology 9 , W. Wood, Academic Press, NY, 87, 1966 4.  Avigad, G., Amaral, D., Asenio, G., and Horecker, B.: Galactodialdolase Production with an Enzyme from the Mold Polyporus circinatus ,Biochem Biophys Res Commun 4, 474, 1961 5. Avigad, G.: Oxidation Rates of Some Desialylated Glycoproteins by Galactose Oxidase, Arch Biochem Biophys 239 , 531, 1985 6. Blumberg, W., Horecker, B., Kelly-Falcoz, F., and Peisach, J.: The Role of Copper in Galactose Oxidase, Biochim Biophys Acta 96 , 336, 1965 7. Cleveland, L. Coffman, R., Coon, P., and Davis, L.: An Investigation of the Role of the Copper in Galactose Oxidase, Biochem 14 , 1108, 1975 8. Cooper, J., Smith, W., Bacila, M., and Medina, H.: Galactose Oxidase from Polyporus circinatus ,J Biol Chem 234 , 445, 1959
 
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Frings, C., and Pardue, H.: An Automatic Spectrophotometric Method for the Specific Enzymatic Determination of Galactose in Whole Blood and Plasma, Anal Chem 36 , 2477, 1964
 
Guilbault, G., Brignac, P., and Juneau, M.: New Substrates for the Fluorometric Determination of Oxidative Enzymes, Anal Chem 40 , 1256, 1968
 
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Marwedel, B., and Kurland, R.: Fluoride Ion as an NMR Relaxation Probe of Galactose Oxidase-Substrate Binding, Biochim Biophys Acta 657 , 495, 1981
 
Myers, R., Ullman, M. Ventura, R., and Yates, A.: A High Performance Liquid Chromatography Method for the Analysis of Glycosphingolipids Using Galactose Oxidase/(NaBH 4-H3 Labeling of Intact Cells and Synaptosomes, Anal Biochem 192 , 156, 1991
 
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Roberts, G., and Gupta, S.: Use of Galactose Oxidase in the Histochemical Examination of Mucus-secreting Cells, Nature 207 , 425, 1965
 
Robinson, J., and Pierce, J.: Studies on Inherited Variants of Blood Proteins. III. Sequential Action of Neuraminidase and Galactose Oxidase on Transferrin B 1-2B2, Arch Biochem Biophys 106 , 384, 1964
 
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Sempere, J., Gancedo, C., and Asensio, C.: Determination of Galactosamine and N-Acetylgalactosamine in the Presence of Other Hexosamines with Galactose Oxidase, Anal Biochem 12 , 509, 1965
 
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Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.