Technical Data
G1044-51
Galectin-3 (GAL3, Carbohydrate Binding Protein 35, CBP35, Galactose Specific Lectin 3, Galactoside Binding Protein, GALBP, IgE Binding Protein, Laminin Binding Protein, Lectin Galactose Binding Soluble 3, LGALS3, MAC2)
Description:
Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions and may in fact be a broad-spectrum biological response modifier. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Among its numerous proposed functions, Galectin 3 may be a component of a cell-growth regulating system given its elevated expression and nuclear localization in proliferating cells. It has also been found to be a major non-integrin laminin-binding protein suggesting a role in cell adhesion. One of its more interesting proposed functions is as an inhibitor of apoptosis. Galectin 3 shares an NWGR motif with Bcl-2 which has been shown to be an imjportant region in Bcl-2 for suppression of apoptosis. Galectin 3 has been shown to interact with Bcl-2 which may account for the ability of Galectin 3 to suppress programmed cell death in anti-Fas treated Jurkat cells transfected with Galectin 3. In contrast to Galectin 3, Galectin 1 actually induces apoptosis when added to cultures of activated human T cells.

Applications:
Suitable for use in Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:100-1:2000
Immunohistochemistry: 1:10-1:500
Immunohistochemistry: (Paraffin): 6ug/ml
Western Blot: 1ug/ml. Detects a ~26kD protein representing Galactin 3 from Jurkat cells transfected with human Galactin 3.
Immunofluorescence (IC): 1:10-1:500
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat Olfactory bulb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14i288Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Full length recombinant human Galectin 3. Epitope: aa1-58 of Galectin 3.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human Galectin 3 at ~26kD. Species Crossreactivity: rat, mouse and rabbit. Immunohistochemistry staining of Galectin 3 in rat olfactory bulb yields a pattern consistant with nuclear and plasma membrane staining.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Biochemistry, 35: 6073-6079, 1996 2.:Proc. Natl. Acad. Sci., 93: 6737-6742, 1996 3. Biochemistry, 35: 6073-6079, 1996.