		Technical Data
G2034-30	Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372)

Description:
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot.
Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:1000
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Optimal dilution to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6D410	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~1mg/ml (after reconstitution)

Immunogen:

Giantin from Caco-2 cell golgi membrane fraction.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.

Specificity:

Recognizes monkey giantin. Species Crossreactivity: human, mouse, rat, goat, sheep, canine, porcine, mammalian and avian cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa: A.D. Linstedt and H.P. Hauri; Mol. Biol. Cell 4, 679 (1993)2. Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995) 3. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol. 142, 377 (1998) 4. Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004)