Technical Data
G2034-30
Giantin (Macrogolgin, Golgi Complex-associated 372kD Protein, GCP372)
Description:
Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not recommended for Western Blot.
Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:1000
Immunoprecipitation: 2ul
Immunohistochemistry: Frozen sections
Optimal dilution to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16D410Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Giantin from Caco-2 cell golgi membrane fraction.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
Specificity:
Recognizes monkey giantin. Species Crossreactivity: human, mouse, rat, goat, sheep, canine, porcine, mammalian and avian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa: A.D. Linstedt and H.P. Hauri; Mol. Biol. Cell 4, 679 (1993)2. Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995) 3. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol. 142, 377 (1998) 4. Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004)