Technical Data
G2035-07Q
Git1 (ARF GTPase-activating protein GIT1)
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Technical Data |
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G2035-07Q |
Git1 (ARF GTPase-activating protein GIT1) |
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Description: ARF GTPase-activating protein GIT1 (Git1) is a multidomain protein belonging to the ARF family of small GTPases that is comprised of several protein interacting domains including: three Ankyrin repeats (ANKs), an Arf-Gap domain, a C-terminal GRK-binding domain, and a spa2 homology domain. Git1 is thought to have a role as a signaling pathway coordinator, functioning to regulate adhesion, cytoskeletal organization, and vesicle trafficking. It is also a component of several cytoplasmic complexes that contain ARHGEF6, PAK1, and PXN. In brain receptors, Git1 forms a complex with AMPA receptors, GRIP, and liprin-alpha, that is crucial to AMPA receptor targeting and the organization of synapses. Git1 expression is observed in a wide variety of tissues, with the most notable expression in the testis. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5ug/ml detects Git1 on 10ug of rat brain membrane tissue lysate. Optimal dilutions to be determined by the researcher. Positive Control: Rat brain membrane tissue lysate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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