Technical Data
Git1 (ARF GTPase-activating protein GIT1)
ARF GTPase-activating protein GIT1 (Git1) is a multidomain protein belonging to the ARF family of small GTPases that is comprised of several protein interacting domains including: three Ankyrin repeats (ANKs), an Arf-Gap domain, a C-terminal GRK-binding domain, and a spa2 homology domain. Git1 is thought to have a role as a signaling pathway coordinator, functioning to regulate adhesion, cytoskeletal organization, and vesicle trafficking. It is also a component of several cytoplasmic complexes that contain ARHGEF6, PAK1, and PXN. In brain receptors, Git1 forms a complex with AMPA receptors, GRIP, and liprin-alpha, that is crucial to AMPA receptor targeting and the organization of synapses. Git1 expression is observed in a wide variety of tissues, with the most notable expression in the testis.

Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml detects Git1 on 10ug of rat brain membrane tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain membrane tissue lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k11C240Affinity Purified
100ug-20CBlue IceRatMouse
Recombinant protein corresponding to rat Git1.
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Recognizes rat Git1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.