Technical Data
G2036-05A
Glial Derived Neurotrophic Factor (GDNF) (Biotin)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: Using 100ul/well antibody solution, a concentration of 0.15-0.3ug/ml of this antibody is required. When used in conjunction with compatible secondary reagents, allows the detection of 0.2ng/well of recombinant hGDNF.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagent,s the detection limit for recombinant hGDNF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.05mg/ml
Immunogen:
Highly purified, recombinant human Glial Derived Neurotrophic Factor (GDNF)
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4. Conjugated to Biotin. Reconstitute to 50ug/ml with sterile PBS containing 0.1% BSA.
Specificity:
Recognizes human Glial Derived Neurotrophic Factor (GDNF).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.