Technical Data
G3900-01J
GLUT 1 (Glucose Transporter 1)
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Technical Data |
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G3900-01J |
GLUT 1 (Glucose Transporter 1) |
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Description: Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 2-4ug/ml for 2hrs Immunohistochemistry (Formalin/paraffin): 1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. If staining is too light, use lower dilution or longer time. If staining is too strong, use higher dilution or shorter time. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Rat brain lysate, breast carcinoma, squamous cell carcinoma, non-small-cell lung carcinoma, esophageal carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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