Technical Data
GLUT 1 (Glucose Transporter 1)
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 2-4ug/ml for 2 hours
Immunohistochemistry (Formalin/paraffin): 1:200 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. If staining is too light, use lower dilution or longer time. If staining is too strong, use higher dilution or shorter time.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Rat brain lysate, breast carcinoma, squamous cell carcinoma, non-small-cell lung carcinoma, esophageal carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
250ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the C-terminal of human GLUT-1. Cellular Localization: Membrane and focal cytoplasmic. MW of Antigen: 55kD, 45kD depending on the extent of glycosylation. Epitope: C-terminal
Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Recognizes human GLUT-1. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.