Technical Data
G3900-15
GLUT 2 (Glucose Transporter, Insulin Regulatable)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, Mol. Wt. 40-60 kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75 kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 using chemiluminescent detection.
ELISA: 1:10,000-1:50,000. G3900-15F Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
A 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter. Conjugated to KLH. Species homology sequence: 93% mouse, human and 84% in ovine.
Purity:
Serum
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes rat Glut-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thorens, B, et al (1988) Cell, 55, 281-290; 2. Asano, T et al (1989) Nucleic Acid Res. 17, 6386; Suzue, K., et al (1989) Nucleic Acid Res. 17, 10099.