Technical Data
G3900-15D
GLUT 2 (Glucose Transporter, Insulin Regulatable)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-13, 40-80kD) and sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using Chemiluminescence technique.
Immunohistochemistry: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
G3900-15F: GLUT 2, Rat (Glucose Transporter, Insulin Regulatable)

Recommended Tissues:
Liver, intestine, kidney, and beta-pancreatic islet cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide corresponding to 16aa near the C-terminal, cytoplasmic domain of rat Glut-2 (KLH). Subcellular Location: multi-pass membrane protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes rat Glut-2. Species Sequence Homology: mouse and human-93%, ovine-84%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Thorens, B, et al (1988) Cell, 55:281-290. 2. Asano, T et al (1989) Nucleic Acid Res. 17:6386. 3. Suzue, K., et al (1989) Nucleic Acid Res. 17:10099.
Citations:
1. Lee, S.H. (2003) J. Histochem. Cytochem. 51:1005-1015. 2. Osswald, C. (2005) Mol. Cell. Biol. 25:78-87. 3. Harrison, K.A. (1999) Nature Genetics, 23:71-75.
4. Gorogawa S-I (2004) Biochem. Biphys. Res. Commun. 319, 1159-1170. 5. Brissova M, K, 2002, JBC 277, 11225-11232. 6. Hori Y, 2002, PNAS, Dec 2002; 99: 16105 - 16110. 7. Rutjes, N 2002, Kidney International 62, 832. 8. Sumazaki R, 2004, Nature Genetics36, 83-87. 9. Rodriguez SM, 2004 J Anim Sci, 82: 3015 - 3023.