Technical Data
G3900-15M
GLUT 2 (Glucose Transporter, Insulin Regulatable)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 13, Mol. Wt. 40-80kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 2 provides glucose to the liver and pancreatic cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000-1:10,000. G3900-15L Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL. This antibody identified a major band at about 53-61kD in various tissues (1).
Immunohistochemistry: 2-10ug/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
G3900-15L: GLUT 2, Human Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 14aa synthetic peptide sequence near the C-terminal extracellular domain. (KLH). Species Sequence Homology: rat - 64%, mouse - 57%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human GLUT 2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fukumoto, H., et al., Proc. Natl. Acad. Sci. 85: 5434-5438 (1988). 2. Thorens, B., et al., Cell 55: 281-290 (1988). 3. Asano, T., et al., Nucleic Acid Res. 17: 6386 (1989). 4. Suzue, K., et al., Nucleic Acid Res. 17: 10,099 (1989).
Citations:
1.Garcia MDL 2003 J. Neurochemistry 86, 3, 709-724 Hypothalamic ependymal-glial cells express the glucose transporter GLUT2, a protein involved in glucose sensing- IF, IHC. 2. Hori Y 2005 PLoS Medicine, Apr2005, Vol. 2 Issue 4, p347-356 Differentiation of Insulin-Producing Cells from Human Neural Progenitor Cells.-IF. 3. Mariggio S 2007 FEBS Letters, Volume 580, Issue 30, 22 December 2006, Pages 6789-6796 Molecular characterization of a glycerophosphoinositol transporter in mammalian cells-IHC.