Technical Data
G3900-24A
GLUT 3 (Glucose Transporter)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT 1 to GLUT 7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662aa; ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting.
GLUT isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT 1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. GLUT 2 provides glucose to the liver and pancreatic cells. GLUT 3 is the main transporter in neurons, whereas GLUT 4 is primarily expressed in muscle and adipose tissue and regulated by insulin.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 (ECL); Detects a major band at ~45kD.
ELISA: 1:10,000-50,000; Coat plates at 1ug/ml with Control Peptide.
Optimal dilutions to be determined by the researcher.

Control Peptide:
G3900-24D: GLUT 3, Human (Glucose Transporter)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to 12aa sequence in the C-terminus of human glucose transporter-3 (hGLUT 3) (KLH coupled).
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide and 40% glycerol.
Specificity:
Recognizes human GLUT 3 (Glucose Transporter). Species Crossreactivity: Does not recognize mouse/rat GLUT 3. For mouse and rat samples use G3900-25 or G3900-26.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kayano, T., et al., J. Biol. Chem. 263: 15245-15248 (1988). 2. Gould, et al., Diabetologia 35: 304-309 (1992). 3. Nagamatsu, et al., J. Biol. Chem. 267: 467-472 (1992). 4. Brant, et al., Biochem. Soc. Trans. 20: 235S-236S-403 (1992). 5. Baldwin, S.A., Biochim. Biophys. Acta 1154: 17-49 (1993). 6. Mueckler, M., Eur. J. Biochem. 219: 713-725 (1994).