Technical Data
G3900-97A
GLUT 12 (Glucose Transporter)
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Technical Data |
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G3900-97A |
GLUT 12 (Glucose Transporter) |
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Description: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT-1 to GLUT-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1: 662aa, ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. GLUT isoform differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT-12 (human 617aa, monkey 621aa, ~ 50kD; ~30% homology with GLUT-4 and 40% with GLUT-10) is expressed in skeletal muscle, adipose tissue, and small intestine. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:10,000-1:50,000 Optimal dilutions to be determined by the researcher. Recommended Control Peptide: G3900-97: GLUT 12, Human, Control Peptide (Glucose Transporter) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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