Technical Data
GLUT 12 (Glucose Transporter)
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT-1 to GLUT-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1: 662aa, ~75kD). Individual members of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. GLUT isoform differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT-12 (human 617aa, monkey 621aa, ~ 50kD; ~30% homology with GLUT-4 and 40% with GLUT-10) is expressed in skeletal muscle, adipose tissue, and small intestine.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000 (ECL).
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
G3900-97: GLUT 12, Human, Control Peptide (Glucose Transporter)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceHumanRabbit
Not Determined
Synthetic peptide consisting of 15aa sequence near the cytoplasmic, C-terminus of human Glut-12 (KLH). Species Sequence Homology: monkey: 100%; mouse: 71%; rat: 69%.
Supplied as a liquid in PBS, 0.05% sodium azide.
Recognizes human GLUT 12. No significant sequence homology with other GLUTs.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doege, H., et al., Biochem. J. 359: 443-459 (2001).