Technical Data
G3900-97D
GLUT 12 (Glucose Transporter)
Description:
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (GLUT-1 to GLUT-7, MW 40-60kD) and Sodium glucose co-transporter-1 (SGLT-1: 662aa, ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N- and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. GLUT isoform differ in their tissue expression, substrate specificity and kinetic characteristics. GLUT-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells. GLUT-2 provides glucose to the liver and pancreatic cells. GLUT-3 is the main transporter in neurons. GLUT-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. GLUT-5 transports fructose in intestine and testis. GLUT-6/SLC2A6, originally described as gLUT-9 has been reassigned as GLUT-6. GlLUT-6 (human: 507aa, ~ 48kD, chromosome 9q34) is most closely related to GLUT-8 (~45% homology). It is highly expressed in brain, spleen, and peripheral leukocytes. GLUT-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. GlLUT-8 is found in adult testis and placenta.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml. Control peptide (Cat #G3900-97) can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide consisting of 15aa sequence near the cytoplasmic, C-terminus of Human Glut-12 (KLH coupled).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity:
Recognizes human GLUT 12. No significant sequence homology with other GLUTs. Species Sequence Homology: monkey: 100%; mouse: 71%; rat: 69%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doege, H., et al., Biochem. J. 359: 443-459 (2001).