Technical Data
G4000-01D
Glutamate Decarboxylase 65 (GAD65)
Description:
Gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is am amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).

Applications:
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000- 1:5000. ~65kD band in rat brain.
ELISA: 1:10,000-1:100,000 using 50-100ng UCP2 control peptide/well.
Immunoprecipitation: 10ul.
Optimal dilutions to be determined by the researcher.

Control Peptide: G4000-01A: Glutamate Decarboxylase 65, Rat, Control Peptide (GAD65)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H23Ascites
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceRatMouse
Concentration:
Not determined.
Immunogen:
An 18aa peptide sequence near the N-terminus of rat GAD65.
Purity:
Ascites.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes rat Glutamate Decarboxylase 65 (GAD65). Species Seqeunce Homology: porcine: 100%; mouse: 94%; human and monkey: 83% (16/18aa).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Erlanger MG et al (1991) Neuron 7, 91-100 2. Dirkx R et al (1995) J Biol. Chem. 270, 2241; Solimena, M (unpublished results on file).