Technical Data
Glutamate Transporter, Neuronal (EAAC1, EAAT3)
The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preadsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining

Suitable for use in Immunohistochemistry. Not recommended for use in Western blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:4000-1:8000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material and the use of triton X-100 in the block only is recommended.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceRatGoat
Not Determined
Synthetic peptide corresponding to the carboxy-terminus of the cloned rat EAAC1. Species sequence homology: human, rabbit and monkey.
Supplied as a liquid. No preservatives added.
Recognizes rat Neuronal Glutamate Transporter (EAAC1; also called EAAT3). Species Crossreactivity: mouse and guinea pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nature (1992) 360:467471. 2. Neuron (1994) 13:713725. 3. J. Physio. (1995) 482:7S-17S. 4. NeuroReport (1995) 6:23572362. A good reference for the comparative staining of EAAC1, GLAST and GLT-1 is: 5. Neuron (1994) 13:713725.