Technical Data
Glutathione is a tripeptide (gama-glutamylcysteinylglycine) molecule found in most mammmalian cells. It functions in basic metabolic processes such as serving as a sulfydryl buffer to maintain redox within the cell, participating in the transport of amino acids and detoxifying hydrogen and organic peroxides. Glutathione depletion can inhibit Th-1 associated cytokine production and favors a Th-2 associated immune response. Glutathione deficiency has also been linked to decreased patient survival in HIV-infected individuals. Overexpression of apoptotic protein Bcl-2 causes relocalization of glutathione from the cytoplasm into the nucleus where glutathione is thought to alter the redox and block caspase activity.

Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000. Under non-reducing conditions
ELISA: 1:1000
Immunoprecipitation: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a1V022Affinity Purified
100ug-20CBlue IceMouse
Glutathione-protein complexes
Purified by Protein A affinity chromatography
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Recognizes Glutathione. Reacts with Glutathione-protein complexes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amara, A., et al., BrainRes., 659, 237-242 (1994).