Technical Data
G8123
Glutathione
Description:
Glutathione is a tripeptide (gama-glutamylcysteinylglycine) molecule found in most mammmalian cells. It functions in basic metabolic processes such as serving as a sulfydryl buffer to maintain redox within the cell, participating in the transport of amino acids and detoxifying hydrogen and organic peroxides. Glutathione depletion can inhibit Th-1 associated cytokine production and favors a Th-2 associated immune response. Glutathione deficiency has also been linked to decreased patient survival in HIV-infected individuals. Overexpression of apoptotic protein Bcl-2 causes relocalization of glutathione from the cytoplasm into the nucleus where glutathione is thought to alter the redox and block caspase activity.

Applications:
Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000. Under non-reducing conditions
ELISA: 1:1000
Immunoprecipitation: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a1V022Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Glutathione-protein complexes
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Specificity:
Recognizes Glutathione. Reacts with Glutathione-protein complexes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Amara, A., et al., BrainRes., 659, 237-242 (1994).