Technical Data
G8950-10A
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
5ug
20ug
Molecular Biology Storage: -20CShipping: RT
GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant Human GM-CSF is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477D.

Amino Acid Sequence:
MAPARSPSP STQPWEHVN AIQEARRLL NLSRDTAAE MNETVEVIS EMFDLQEPT CLQTRLELY KQGLRGSLT KLKGPLTMM ASHYKQHCP PTPETSCAT QIITFESFK ENLKDFLLV IPFDCWEPV QE

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.1 ng/ml, corresponding to a specific activity of 1x10e7units/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
N/A

Molecular Weight:
14477D
Source: E. coli
Purity: 98% by HPLC and SDS-PAGE. Endotoxin: 0.1ng/ug (IEU/ug)
Form: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.5. Reconstitute with sterile ddH2O, 0.1% BSA to a concnetration of 0.1-1mg/ml. Do not vortex.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.