Technical Data
G8950-10A
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: RT
GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2-beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors

Recombinant Human GM-CSF is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14,477D..

Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin: 0.1ng/ug (IEU/ug)

Biological Activity:
GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less then 0.1ng/ml, corresponding to a Specific Activity of ~11x10e6IU/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding with sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

CAS Number:
N/A
Source: Recombinant, Human, E. coli
Purity: 98% by RP-HPLC and anion-exchange FPLC, and reducing/non-reducing SDS-PAGE (silver stained).
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder from 2mM sodium phosphate buffer, pH 7.4. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Alenzi FQ, Br J Biomed Sci 2004;61(4):200-5 2. Kamata N, Kutsuna H, Kato T, Int J Hematol 2004 Dec;80(5):421-7 3. Chan RJ, Leedy MB, Voorhorst CS, Blood 2005 Jan 11; 4. Westcott MM, Abi-Habib RJ, Willingham MC, Frankel AE, Mol Cancer Ther 2004 Dec;3(12):1681-9 5. Ren H, Lu D, Zhonghua Xue Ye Xue Za Zhi 1997 Mar;18(3):137-9 6. Marshall JL, Gulley JL, Beetham PK, Doren S, J Clin Oncol 2004 Dec 21.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.