Technical Data
G8950-20
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Mouse (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl
2ug
10ug
Growth Factors, Cytokines Storage: -20°CShipping: RT
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Murine GM-CSF is a 14.0kD protein containing 125 amino acid residues.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells. The ED50 is  0.2ng/ml, corresponding to a specific activity of 5x10e6u/mg.

Responding Cells (partial list): Certain BM Precursor cells. For most in vitro applications, murine GM-CSF exerts its biological activity in the concentration range of 0.05-0.5ng/ml.

Amino Acid Sequence: The sequence of the first five N-terminal amino acids was found to be Met-Ala-Pro-Thr-Arg.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. For long term storage, store desiccated at -20°C. Reconstituted product should be stored in working aliquots at -20°C. Aliquots are stable for 12 months at –20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
14.2kD
 Source: Murine, recombinant protein expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin; 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder. No additives. Reconstitute in sterile 20mM acetic acid to a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Kilinc, M.O. et al., (2006) Exp Mol Pathol. 80:252-261.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.