Technical Data
GM-CSF, Recombinant, Human (Granulocyte-macrophage Colony-stimulating Factor, GM-CSF, Colony-stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF)
Growth Factors, Cytokines Storage: -70CShipping: Dry Ice
Human GM-CSF is a 24kD glycoprotein produced by a variety of cell types, including T and B lymphocytes, macrophages, keratinocytes, eosinophils, neutrophils, and endothelial cells in response to cytokine or inflammatory stimuli. GM-CSF was initially identified on the basis of its capacity to stimulate the clonal proliferation of myeloid precursors in vitro. GM-CSF promotes a Th1 biased immune response, allergic inflammation, and the development of autoimmunity. GM-CSF are particularly effective as antitumor vaccines. GM-CSF regulates multiple biological activities through activation of the GM-CSF receptor (GMCSFR), a member of the type I cytokine receptor group comprising a and b subunits.

Recombinant corresponding to aa18-144, from human GM-CSF, expressed in E.coli.

Molecular Weight:

Endotoxin Level:
1EU/1ug (LAL)

Specific Activity:
The ED50 for this effect is typically 10-40pg/ml. Measured in a cell proliferation assay using the human growth factor-dependent cell line TF-1.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in PBS, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Conti L and Gessani S., 2008, Immunobiology 213:859-870. 2. Waller EK., 2007, Oncologist 12:22-6.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.