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Technical Data |
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G8951-26R |
Granulocyte Macrophage Colony Stimulating Factor, Recombinant, Human (GM-CSF, Burst Promoting Activity, CMCSF, Colony Stimulating Factor 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, Molgramostin, Pl |
5ug 10ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Dry Ice |
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GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. Biological Activity: Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.1ng/ml. Specific Activity: 1x10e7 IU/mg. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 12 months at -70°C. Reconstitute by adding sterile ddH2O or aqueous buffer containing 0.1% BSA. Aliquot and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 14kD |
Source: Escherichia coli Purity: ~96% by SDS-PAGE and HPLC analyses. Endotoxin: 1EU/ug a (LAL). Concentration: ~1mg/ml Form: Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute in sterile ddH2O or aqueous buffer containing 0.1% BSA to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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