		Technical Data
G8965-04E2	Green Fluorescent Protein (GFP) (FITC)

Description:
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm)and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence.The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.

Applications:
Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: Tested on prokaryotic (E. coli) and eukaroytic (CHO cells) expression systems. Detected GRP containing inserts.
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Goat

Concentration:

~1mg/ml

Immunogen:

Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish, Aequorea victoria.

Purity:

Purified

Form

0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2]

Specificity:

Recognizes GFP and its variants. Species Crossreactivity: Does not react with human, mouse and rat proteins.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970).