Technical Data
Green Fluorescent Protein (GFP) (FITC)
GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm)and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence.The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications.

Suitable for use in FLISA, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: Tested on prokaryotic (E. coli) and eukaroytic (CHO cells) expression systems. Detected GRP containing inserts.
Immunohistochemistry: Frozen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceGoat
Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish, Aequorea victoria.
0.02M Potassium phosphate, 0.15M sodium chloride, [pH7.2]
Recognizes GFP and its variants. Species Crossreactivity: Does not react with human, mouse and rat proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The TH & Feltkamp TE Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology 18:865-73 (1970).