Technical Data
G8965-06
Green Fluorescent Protein (GFP)
Description:
Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml detects GFP from 50ug of rat brain lysate containing 25ng of purified GFP. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system.
Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYPurified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceChicken
Concentration:
~1mg/ml
Immunogen:
His-tagged green fluorescent protein of Aequorea victoria.
Purity:
Purified by ammonium sulfate precipitation
Form
Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity:
Recognizes green fluorescent protein (GFP), Mr 30kD, and GFP-fusion proteins. Species Crossreactivity: Vertebrates
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Gerdes, H. H. and Kaether, C., FEBS Letters 389: 4447 (1996) Kain, S.R., et al., BioTechniques 19: 650655 (1995) Davis, I. et al. Developmental Biology 170: 726-729 (1995)