Technical Data
G8965-06
Green Fluorescent Protein (GFP)
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Technical Data |
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G8965-06 |
Green Fluorescent Protein (GFP) |
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Description: Green fluorescent protein (GFP) is a 29kD fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488nm), GFP strongly fluoresces (Emission max: 507nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects GFP from 50ug of rat brain lysate containing 25ng of purified GFP. Also detected a GFP-fusion protein from a transfected E. coli cell lysate. Rat brain lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with G8965-06 (1ug/ml). Proteins were visualized using a rabbit anti-chicken secondary antibody conjugated to HRP* and a chemiluminescence detection system. Immunocytochemistry: Reported to show positive immunostaining in cells transfected with an expression vector encoding GFP. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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