Technical Data
G8965-10
Green Fluorescent Protein (GFP)
Description:
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509nm) when excited with blue or UV light.

Applications:
Suitable for use in Immunoblot and ELISA. Other applications not tested.

Recommended Dilution:
Immunoblot: 1:250-1:500 using colorimetric detection
(Peroxidase/TMB), with higher dilution possible using more sensitive detection methods.
ELISA: 1:400-1:500
Optimal dilutions to be determined by the researcher.

Note:
While this antibody has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, use highly-purified secondary antibodies in indirect detection systems.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRabbit
Concentration:
~0.05mg/ml
Immunogen:
Highly purified native GFP from Aequorea victoria
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 100mM PBS, pH 7.2, before the addition of glycerol to 40%. Contains traces of citrate buffer, tris buffer and sodium azide.
Specificity:
Reactive with GFP from both native and recombinant sources. Minimal crossreactivity with E. coli.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Togashi, H., et al., Neuron 35: 77-89 (2002). 2. Ashby, M.C., et al., Journal of Neuroscience 24: 5172-5176 (2004).