Technical Data
G8965-10E
Green Fluorescent Protein, Recombinant (GFP)
50ug
300ug
300ug
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Technical Data |
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G8965-10E |
Green Fluorescent Protein, Recombinant (GFP) |
50ug 300ug |
| BioSeparation? | Storage: -20°CShipping: Blue Ice |
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Full-length Green Fluorescent Protein with N-terminal HIS-tag (6X), recombinant, expressed in E. coli. GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Accession Number: AAA27722 Applications: Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~28kD |
Source: E. coli Purity: Purified using Ni-NTA agarose (~70%). Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 20% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Prasher, D.C., et al., Gene 111: 229, 1992.
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