Technical Data
G8965-12A
Green Fluorescent Protein (GFP) (FITC)
Description:
Anti-GFP assayed in a sandwich ELISA is well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated G8965-12A was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated G8965-12A relative to the fluorescence of GFP alone.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence 1:500-1:2500
ELISA: Sandwich or capture
Optimal dilution determined by the researcher.

Absorption Wavelength: 495nm

Emission Wavelength: 528nm

Fluorochrome/Protein Ratio:
2.9 moles FITC per mole of Goat IgG

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceGoat
Concentration:
~1mg/ml
Immunogen:
GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2,10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes Green Fluorescent Protein (GFP). Assayed by ELISA for direct binding of antigen, recognizing wild type, recombinant and enhanced forms of GFP. Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-Fluorescein and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.