Technical Data
G9039-54
Guanine Neculeotide Exchange Factor H1 (GEF-H1, Rho/Rac guanine nucleotide exchange factor 2, Proliferating cell nucleolar antigen p40, Lfc)
Description:
GEF-H1 can activate the small GTPase RhoA, but not Rac1 or Cdc42. Rho family GTPases are central regulators of the cytoskeleton. GEF-H1 can associate with different cytoskeletal structures, namely microtubules and the actin cytoskeleton. It has also been proposed to mediate crosstalk between the two types of filaments. Localization of GEF-H1 differs between cell types. In MRC-5 fibroblast cells, GEF-H1 localizes to stress fibers. In epithelial cells, GEF-H1 is associated with apical tight junctions and involved in regulating paracellular permeability of small hydrophilic tracers. Furthermore, its subcellular localization changes in mitotic cells, where endogenous GEF-H1 is concentrated at mitotic spindles. GEF-H1 is capable of binding to the F-actin binding junctional adaptor cingulin. Binding to cingulin inhibits GEF-H1 and results in the downregulation of RhoA and inhibition of G1/S phase transition. In low confluent cultured cells, the localization of GEF-H1 is predominantly cytoplasmic. With increasing density of the cells, free GEF-H1 is sequestered at the tight junctions by cingulin.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:50
ELISA: 1:50
Immunoprecipitation: 1:50
Immunohistochemistry: frozen
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1B4/7Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceCanineMouse
Concentration:
~0.1mg/ml
Immunogen:
Raised against a Guanine nucleotide exhange factor H1 protein fraction isolated from detergent extracts of MDCK cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1mg/m l0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes canine guanine nucleotide exchange factor H1 (GEF-H1). Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Benais-Pont, G et al; Identification of a tight junction-associated guanine nucleotide exchange factor
that activates Rho and regulates paracellular permeability. J Cell Biol 2003, 160: 729 2. Aijaz, S et al; Binding of GEF-H1 to the tight-junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition. Dev Cell 2005, 8: 777.