		Technical Data
H1000-50B	HA-1 (HLA-HA1, HMHA1, KIAA0223, Minor Histocompatibility Antigen HA-1, mHag HA-1, Minor Histocompatibility Protein HA-1)

Description:
The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa883-912 from the center region of human HA-1.

Purity:

Purified by Protein A and peptide affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human HA-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gillespie,G.,et.al., Hematol. J. 1(6), 403-410 (2000).