Technical Data
Hamartin (TSC1, Tuberous Sclerosis 1)
Tuberous sclerosis complexes-1 (TCS1 also known as Hamartin) is a tumor suppressor that form a complex with TSC2 (Tuberin. This complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (2). Recently it has been shown that TSC1/Hamartin is localized to the centrosome and negatively regulates Plk1 implicating TSC1/hamartin to the regulation of centrosome duplication (3). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:20,000 (MW:150kD)
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Not Determined
A synthetic peptide corresponding to C-terminal residues of human TSC1/Hamartin.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Recognizes human TSC1/Hamartin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Lamb,et al. Nature Cell Biol 2:281-28 2. Manning BD, et al. Molec. Cell 10:151-162, 2002. 3. Astrinidis A, Senapedis W, et al. Hum Mol Genet 15:287-297, 2005.