Technical Data
H1779-01B
Hamartin (TSC1, Tuberous Sclerosis 1)
Description:
Tuberous sclerosis complexes-1 (TCS1 also known as Hamartin) is a tumor suppressor that form a complex with TSC2 (Tuberin. This complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (2). Recently it has been shown that TSC1/Hamartin is localized to the centrosome and negatively regulates Plk1 implicating TSC1/hamartin to the regulation of centrosome duplication (3). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:20,000 (MW:150kD)
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG6k196Supernatant
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
A synthetic peptide corresponding to C-terminal residues of human TSC1/Hamartin.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human TSC1/Hamartin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Lamb,et al. Nature Cell Biol 2:281-28 2. Manning BD, et al. Molec. Cell 10:151-162, 2002. 3. Astrinidis A, Senapedis W, et al. Hum Mol Genet 15:287-297, 2005.