Technical Data
H1827-51
HDAC1 (Histone Deacetylase 1)
Description:
Histone deacetylase 1 (HDAC1), is responsible for the deacetylation of lysine residues on the N-terminal portion of core histones H2A, H2B, H3, and H4. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multi-protein complexes.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Chromatin Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.T.12Affinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to aa467-482, C-EEKPEAKGVKEEVKLA, of human HDAC1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human HDAC1, Mr 60kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Doetzlhofer, A., et al., Mol. Cell. Biol. 19: 5504-5511 (1999). 2. Lagger, G., et al., Mol. Cell. Biol. 23: 2669-2679 (2003). 3. Gaughan, L., et al., J. Biol. Chem. 277: 25,904-25,913 (2002).