Technical Data
H1827-54
HDAC2 (Histone Deacetylase 2, Tanscriptional Regulator Homolog RPD3, HD2)
Description:
HDAC2 (Histone deacetylase 2) belongs to the histone deacetylase family. Histone deacetylases are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3, and H4) via the formation of large multiprotein complexes. Histone deacetylation forms the tagging for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC2 also forms transcriptional repressor complexes by associating with many different proteins, such as YY1, a mammalian zinc-finger transcription factor.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide derived from the carboxyl terminal residues of the human HDAC2 protein (Swiss Prot: Q92769).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes endogenous levels of human HDAC2 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.