Technical Data
Heart Infusion Agar (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components are shown as g/Liter:
Brain Heart Infusion from Solids2.0
Casein Digest Peptone10.0
Peptic Digest of Animal Tissue5.0
Supplemented with blood a medium to culture a wide variety of organisms and observe hemolytic activity of streptococci.

Heart Infusion Agar is prepared from an infusion of beef heart. The first media used for the cultivation of bacteria was prepared from meat infusions. Huntoon showed that pathogenic microorganisms could be grown from infusion agar without enrichments. This medium could be used for mass culture of microorganisms for vaccine production. It was also used for determining the presence and bacterial count of hemolytic streptococci in milk.

Storage and Stability:
Store the sealed bottle containing the dehydrated medium at 4C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Directions: 1. Dissolve 40g per 1L of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Distribute and autoclave for at 121.0C for 15 minutes. 3. Prepare 5 to 10 % blood agar by adding appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50.0C..Storage:Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be stored at 4C and used within a short period of time.

Microbiological Analysis: Neisseria meningitidis ATCC 13090

Appearance: Light beige to beige, homogeneous, free flowing powder.

Solubility: Light to medium yellow beige, trace to light haze

pH: 7.40.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.