Technical Data
Heparan Sulfate, Na Salt, Bovine
Molecular Biology Storage: 4C (-20C Reconstitution)Shipping: Blue Ice
Heparan sulfate, bovine, is specially prepared from bovine kidney, purified by a combination of chromatographic and chondroitinase ABC treatment.

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4)-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS-(1->4)--D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4)alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc(1->4)--D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.
Purity (TLC): Single band
Appearance: White powder
Total Nitrogen: 2.6-3.2%
Total Sulfur: 4.56.0%
Inorganic Sulfur: Not detected
Chlorine: Not detected
Uronic Acid (Carbazol reaction): 36.0-40.0%
Glucosamine: 30.035.0%
Galactosamine: 0.01%
Specific Rotation: +75 to +88
1. A. lanber, T. Hoffman, T. Sanpson, K. Meyer, Biochim. Biophys. Acta., 29:443 (1958). 2. S. Schiller, G. A. Slover, A. Dorfman, J. Biol. Chme. 236:983 (1961). 3. N. Seno, K. Anno, K. Kondo, S. Nagase, S. Saito, Anal. Biochem., 37:197 (1970) 4. W. Schoniger, Mikrochim. Acta., 123 (1955) 5. A. H. Brown : Arch, Biochem., 11:268 (1946) 6. T. Bitter, H. M. Muir, Anal. Biochem., 4:330 (1962).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.