Technical Data
H1890-75
Heparan Sulfate delta (3G10 epitope)
Description:
Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) -D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha- D-GlcNpS- (1->4)--D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha- D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)- alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4) -alpha-D-GlcNpAc (1->4)--D-GlcAp(1->disaccharide sequence that is attached to a serine residue of a core protein through a tetra- saccharide, glucuronosyl->galactosyl-> galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epi- merization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: 1:100-1:200 (0.5-1ug labels ~10e6 cells).
Immunohistochemistry: 1:100-1:200
ELISA: 1:200-1:500
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma SP2/0-Ag14 with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k8.S.222Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Heparitinase-digested medium heparan sulfate proteoglycans from human lung fibroblasts.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human heparan sulfate neo-epitope, generated by digesting heparan sulfate with heparitinase from Flavobacterium heparinum (EC4.2.2.8). The desaturated hexuronate (glucuronate) that is present at the non-reducing end of the heparan sulfate fragments created by the enzyme is critical for the reactivity of the antibody. Does not react with oligosaccharides generated from chondroitin sulfates with bacterial chondroitin ABC or AC lyases or generated from heparan sulfate with heparinase (EC4.2.2.7).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. David, G., et al., J. Cell. Biol. 119: 961-975 (1992). 2. Bai, X.M., et al., J. Histochem. Cytochem. 42: 1043-1054 (1994). 3. Nackaerts, K., et al., Int. J. Cancer (Pred. Oncol.) 74: 335-345 (1997). 4. Fuxe, K., et al., Brain Research 636: 131-138 (1994).