Technical Data
H1890-87A
Heparan Sulfate Proteoglycan, Large
Description:
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulfate, heparin, chondroitin sulfate, dermatan sulfate or keratan sulfate). Two forms of basement membrane heparan sulfate proteoglycan (HSPG) have been identified. One with a large core protein (> 400kD) and one with a small core protein (30kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100-1:1000
Immunohistochemistry (Frozen, paraffin): 1:25-1:200 with avidin-biotinylated horseradish peroxidase complex (ABC) as a detecting reagent.
Optimum dilutions to be determined by researcher.

Hybridoma:
X63Ag8.653 mouse myeloma cells with spleen cells from a Fisher rat.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a4A49Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and Heparan Sulfate Proteoglycan.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes the large Heparan Sulfate Proteoglycan. Reacts with domain IV of the core protein of the large heparan sulfate proteoglycan or perlecan. Reactivity is independent of the galactosaminoglycan moieties. The epitope is therefore not sensitive to heparitinase treatment. Species Crossreactivity: human, mouse, rat and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Couchman, J.R. & Ljubimov, A.V., Matrix 9: 311-321 (1989). 2. Horiguchi, Y., et al., J. Histochem. Cytochem. 37: 961-970 (1989). 3. Ljubimov, A.V., et al., Int. J. Cancer 50: 562-566 (1992). 4. Couchman, J.R., et al., J. Histochem. Cytochem. 43: 955-963 (1995). 5. Tapanadechopone, P., et al., Biochem. Biophys. Res. Commun. 265: 680-690 (1999). 6. Couchman, J.R., J. Cell Biol. 105: 1901-1916 (1987). 7. Tingbo, M.G., et al., Comparative Biochemistry and Physiology 143: 441-452 (2006).