Technical Data
H2005-02
Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro. It may modulate hematopoiesis.
Recombinant Human HGF is a heterodimer, non-glycosylated, polypeptide chain consisting an alpha-chain of 463aa and beta-chain of 234aa having a total molecular mass of 80kD.

Sequence:
Agrees with the seqeunce of native human HGF.

Endotoxin:
0.1ng/ug (IEU/ug) of HGF.

Dimers and Aggregates:
< 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells, was found to be 20-40ng/ml.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 24 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human, recombinant protein expressed by Baculovirus Infected High-5 cells
Purity: 98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).

Form: Supplied as a lyophilized powder. Reconstitute in sterile dH2O, 0.1% HSA or BSA to a concentration > 0.1mg/ml. Can be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Jinnin, M., Ihn, H., Mimura, Y., Asano, Y., Yamane, K., Tamaki, K.: Matrix metalloproteinase-1 up-regulation by hepatocyte growth factor in human dermal fibroblasts via ERK signaling pathway involves Ets1 and Fli1. Nucleic Acids Res. 33(11): 3540-9 (2005, Jun 21). 2. Moriyama, M., Matsumura, H., Watanabe, A., Oshiro, S., Aoki, H., Shimizu, T., Nakai, K., Yamagami, H., Kaneko, M., Shioda, A., Tanaka, N., Arakawa, Y.: Evaluation of serum concentrations of human hepatocyte growth factor during interferon therapy for chronic hepatitis C. Intervirology 48(4): 223-9 (2005, Jul-Aug). 3. Mizuno, S., Nakamura, T.: Prevention of neutrophil extravasation by hepatocyte growth factor leads to attenuations of tubular apoptosis and renal dysfunction in mouse ischemic kidneys. Am. J. Pathol. 166(6): 1895-905 (2005, Jun). 4. Chen, Y., Kobayashi, N., Suzuki, S., Soto-Gutierrez, A., Rivas-Carrillo, J.D., Tanaka, K., Navarro-Alvarez, N., Fukazawa, T., Narushima, M., Miki, A., Okitsu, T., Amemiya, H., Tanaka, N.: Transplantation of human hepatocytes cultured with deleted variant of hepatocyte growth factor prolongs the survival of mice with acute liver failure. Transplantation 79(10): 1378-85 (2005, May 27). 5. Ohda, Y., Hori, K., Tomita, T., Hida, N., Kosaka, T., Fukuda, Y., Miwa, H., Matsumoto, T.: Effects of hepatocyte growth factor on rat inflammatory bowel disease models. Dig. Dis. Sci. 50(5): 914-21 (2005, May). 6. Numata, M., Ido, A., Moriuchi, A., Kim, I., Tahara, Y., Yamamoto, S., Hasuike, S., Nagata, K., Miyata, Y., Uto, H., Tsubouchi, H.: Hepatocyte growth factor facilitates the repair of large colonic ulcers in 2,4,6-trinitrobenzene sulfonic acid-induced colitis in rats. Inflamm Bowel Dis. 11(6): 551-8 (2005, Jun).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.