Technical Data
Hepatocyte Growth Factor, Recombinant, Human (HGF, HGFB, F-TCF, Hepatopoietin A, HPTA, Lung Fibroblast-derived Mitogen, Scatter Factor, SF)
Molecular Biology Storage: -20CShipping: Blue Ice
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell
growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary
epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of
hematopoietic progenitor cells in vitro and may, therefore, also modulating hematopoiesis.

Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 aa and a-chain of 234 having a total molecular mass of 80.0kD. Amino Acid Sequence agrees with the sequence of native human HGF.

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.

Biological Activity:
The ED50 calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml which corresponds to a Specific Activity of 25,000-50,000IU/mg.

Storage and Stability:
Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -20C. Upon reconstitution HGF should be stored at 4C between 2-7 days and for future use below -20C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Source: CHO
Purity: 97.0% as determined by RP-HPLC or SDS-PAGE.
Concentration: As reported
Form: Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% Tween 20, from a concentrated (1mg/ml) solution. Reconstitute with sterile dH2O to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.