Technical Data
H2005-07A
Hepatocyte Growth Factor, Recombinant, Human (HGF, HGFB, DFNB39, F-TCF, Hepatopoeitin-A, HPTA, Scatter Factor, SF)
2ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 463aa of human Hepatocyte Growth Factor expressed in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain.

Molecular Weight:
~80kD

Biological Activity:
The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40nug/ml

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~80kD
Source: Baculovirus
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: Not determined
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kidney volume and plasma hepatocyte growth factor-transforming growth factor beta1 ratio among children with biliary atresia before and after liver transplantation: the reversibility of nephromegaly. Am J Kidney Dis 2005 Nov;46( 5):830-6. 2. Modulation of E-cadherin by hepatocyte growth factor induces aggressiveness of gastric carcinoma. Ann Surg 2005 Nov;242(5):676-83. 3. Arginine-specific gingipains from Porphyromonas gingivalis stimulate production of hepatocyte growth factor (scatter factor) through protease-activated receptors in human gingival fibroblasts in culture. J Immunol 2005 Nov 1;175(9):6076-84. 4. Generation of inhibitory DNA aptamers against human hepatocyte growth factor. DNA Cell Biol 2005 Oct;24(10):624-33. 5. Hepatocyte growth factor as well as vascular endothelial growth factor gene induction effectively promotes liver regeneration after hepatectomy in Solt-Farber rats. Hepatogastroenterology 2005 Sep-Oct;52(65):1393-7. 6. [Development of an innovative therapy for inflammatory bowel disease using recombinant human hepatocyte growth factor to facilitate intestinal mucosal repair]. Nippon Shokakibyo Gakkai Zasshi 2005 Sep;102(9):1139-45.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.