Technical Data
Hepatoma Derived Growth Factor Isoform, Recombinant Human, aa1-100 (HDGF)
Molecular Biology Storage: -20CShipping: Blue Ice
Hepatoma-derived growth factor isoform a, also known as HDGF, belongs to the hepatoma-derived growth factor family. HDGF was originally identified as a secreted mitogen from the human hepatoma cell line Huh-7. This nuclear targeted vascular smooth muscle cell mitogen is a heparin-binding protein that is highly expressed in tumor cells where it stimulates proliferation. HDGF is also reported to be involved in organ development and lung remodeling after injury by promoting proliferation of lung epithelial cells. Recombinant human HDGF was expressed in E. coli and purified by using conventional chromatography techniques.

Recombinant corresponding to human HDGF, expressed in E. coli.

AA sequence:

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~11.5kD (100aa)
Source: E.coli
Purity: ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH7.5, 1mM DTT, 1mM EDTA, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Everett AD., et al. (2003) Curr Drug Tarqets. 4(5):367-71. 2. Everett AD., et al. (2004) Am J Physiol Lung Cell Mol Physiol. 28(6):L1194-201.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.