		Technical Data
H2034-35	HES1 (Hairy Family Helix-loop-helix Transcription Factor)

Description:
HES1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. HES1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and HES1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1:200-1:1000
Immunohistochemistry (Frozen sections): 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Human neural stem cells, Raji cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to aa24-35, TPDKPKTASEHR, of human HES1 (NP_005515).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Recognizes human HES1 (Hairy family) at ~29kD. Species Crossreactivity: rodent.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

US Biological Application References: 1. Wang, X., et al., J. Cancer Res. Clin. Oncol. 136: 989-996 (2010). 2. Burkert, J., et al., J. Pathol. 214: 564-573 (2008).