Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach.
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.
Immunohistochemistry (Frozen and paraffin sections): 1:1500-1:2000 (PAP).
Immunofluorescence: 1:300-1:600 with overnight incubation at 4°C.
Optimal dilutions to be determined by the researcher.
Recommended Positive Control:
Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not Determined|
|Histamine, conjugated to BSA|
|Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH2O Further dilutions should be made in PBS, 1% BSA, 0.1% sodium azide.|
|Recognizes Histamine. Absorption with 10-100ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not. Species Crossreactivity: human and rat|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Hakansson R et al. Histamine in endocrine cells in the stomach. A survey of several species using a panel of histamine antibodies. Histochemistry 86, 5-17 (1986) 2. Axelson J et al: Enhanced hyperplasia of gastric enterochromaffinlike cells in response to omeprazole-evoked hypergastrinemia in rats with portacaval shunts. Gastroenterology 99, 635-640 (1990) 3. Lönroth H et al: Histamine containing endocrine cells in the human stomach. Gut 31, 383-388 (1990) 4. Johansson O et al. Histamine immunohistochemistry: a new and highly sensitive method for studying cutaneous mast cells. Histochem J 24, 283-187 (1992)|