Technical Data
H5102
Histidine Decarboxylase (HDC)
Description:
Histidine decarboxylase is the enzyme catalyzing the conversion of histidine into histamine. HDC can be found in the histamine secreting ECL cells of some species as well as in mast cells.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence microscopy. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen, paraffin
Immunofluorescence Microscopy: 1:800-1:1200. Incubate overnight at 4°C.
Optimal dilutions to be determined by the researcher.

Positive Control:
Stefanini-fixed frozen sections of rat fundus.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceGuinea pig
Concentration:
Not Determined
Immunogen:
Recombinant histidine decarboxylase produced in E. coli.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 50-100ul sterile ddH20. Dilute further in 0.1M PBS, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes Histidine Decarboxylase. Species Crossreactivity: human, rat, mouse, canine, frog and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Gaykema, R.P.A. and Goehler, L. E. (2010) Brain, Behavior, and Immunity doi:10.1016/j.bbi.2010.11.005 1. Dartsch C et al, Histochem J 31, 507-514 (1999). 2.Hunyadi B et al, Endocrinology139, 4404-4415 (1998). 3. Dartsch C. et al., Int. J. Biochem.& Cell. Biol. 30, 773-782 (1998). 4. Zhao C.-M. et al., Cell Tissue Res. 290, 539-551 (1997).