Technical Data
H5110-07
Histone H1 (F1), Lysine Rich
10mg
25mg
25mg
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Technical Data |
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H5110-07 |
Histone H1 (F1), Lysine Rich |
10mg 25mg |
| Molecular Biology | Storage: 4°CShipping: RT |
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Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature. Molecular Weights of Histones: Lysine Rich (H1, f1): ~ 31,500 Slightly Lysine Rich (H2a, f2a2): 14,004 Slightly Lysine Rich (H2b, f2b): 13,774 Arginine Rich (H3, f3): 15,324 Arginine Rich (H4, f2a1): 11,282 Description: Histone subclass F1 Specificity: SDS-PAGE Analysis: Doublet at ~31kD (GE Healthcare PhastGel Homogenous 20%) Instructions for Use: Most soluble in an acidic buffer such as 0.9M acetic acid at 1mg/ml. (Some assay systems may require more or less dilute histones.) Allow sufficient time for complete dissolution. Do not store diluted histones, as they will lose activity in solution. Prepare histone solution immediately before use. Storage and Stability: Store lyophilized histones desiccated at 4°C. Reconstitute per reconstitution instructions. Do not store reconstituted histones. Histones lose activity in solutions. Further dilutions can be made in assay buffer. Molecular Weight: 31.5kD |
Source: Calf thymus Purity: Purified Form: Supplied as a white powder Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Bradbury, E.M.: Histone Nomenclature in the Structure and Function of Chromatin, (Fitzsimmins, D.W., and Walstenholme, G.E.W., eds., American Elsevier, New York ) CIBA Foundation Symposium 28: 4 (1975). 2. Elgin, S.C.R. and Weinbrauh, H.: “Chromosomal Proteins and Chromatin Structure”, Annual Review of Biochemistry, p.725, Annual Reviews, Palo Alto p.725 (1975). 3. Johns, E.W. and Butler, J.A.V.: “Further Fractionations of Histones from Calf Thymus”, Biochem. J82: 15 (1962).
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