Technical Data
H5110-14A1
Histone H3, acetylated (Lys9)
Description:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4) is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23 (2,3). Acetylation at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Staining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Chromatin Immunoprecipitation: 1:50
Immunoprecipitation: 1:25
Immunofluorescence (IF-IC): 1:400
Immunohistochemistry (paraffin): 1:800. Recommend antigen retrieval by heating in citrate buffer.
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG7H26Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated.
Purity:
Supernatant
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.15M sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of human histone H3 at ~17kD only when acetylated on Lys9. Does not crossreact with other acetylated histones. Species Crossreactivity: mouse, rat, monkey, zebrafish, C. elegans and S. cerevisiae.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545-579. 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-17641. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45. 4. Cheung, P. et al. (2000) Cell 103, 263-271. 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem. Biol. 9, 1167-1173.6. Jaskelioff, M. and Peterson, C.L. (2003) Nat. Cell Biol. 5, 395-399.7. Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701-713. 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360. 9. Goto, H. et al. (1999) J. Biol. Chem. 274, 25543-25549. 10. Preuss, U. et al. (2003) Nucleic Acids Res. 31, 878-885. 11. Dai, J. et al. (2005) Genes Dev. 19, 472-488.