		Technical Data
H6276-10A	HNE (4-Hydroxy-2-nonenal)

Description:
4-hydroxy-2-nonenal (4-hydroxynonenal, HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml detects HNE in 4-hydroxynonenal or peroxyynitrite-treated recombinant E. coli DNAK
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2b	9H132	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice		Mouse

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

HNE (4-Hydroxy-2-nonenal) conjugated to KLH

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Specificity:

Recognizes 4-HNE adducts of histidine residues. It does not cross-react with nitrotyrosine, formaldehyde, glutaraldehyde, or 4-hydroxy hexenaldehyde adducts.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.