Technical Data
Hormone Sensitive Lipase, phosphorylated (Ser855) (HSL, Lipase Hormone Sensitive, LHS, LIPE)
Hormone-sensitive lipase (HSL) is a key enzyme in fatty acid mobilization, overall energy
homeostasis, and possibly steroidogenesis.It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1).HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the
adrenals, ovaries, testes, and macrophages (2, 3).HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development.HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis.The HSL protein levels and enzymatic activity in
ITf and STf were reported positively correlated with serum testosterone levels during development (4).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
A phospho-specific peptide corresponding to residues surrounding Serine 855 of human HSL.
Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% glycerol, 0.01% sodium azide and 0.05% BSA.
Recognizes human HSL when phosphorylated at Ser855. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holm C, et al. Science 241(4872):1503-6, 1988 2. Li Z, et al. Genomics 24(2):259-65, 1994
3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000 4. Kabbaj O, et al Biology of eproduction 65:601–612, 2001